Method for administering water soluble drugs, nutrients and other solutes to a mammal

ABSTRACT

Drugs, nutrients and other solutes are administered to fixed and mobile tissue cells of the mammal in an aqueous solution containing about 75-200 mM/L of Na , about 7-75 mM/L K , about 12-28 mM/L HCO3 , about 70-195 mM/L Cl and preferably containing about 3-8 mM/L Mg and about 3-8 mM/L HPO4 and/or SO4 , and/or acetate, and/or gluconate ; the solution having a pH of about 7.4 to 8.4 and an osmolality of about 170-460, preferably about 260-340, more preferably about 290-310, and most preferably 300 milliosmols per liter. The composition can contain other additives, e.g. stabilizing agents, and the solutes to be carried, e.g., antibiotics, vitamins, nutrients, and other solutes.

United States Patent Reynolds *July 29, 1975 [54] METHOD FOR ADMINISTERING WATER 2,224,252 12/1940 Callaway 99/1 AND 2,265,453 12/1941 Schmidt..... 424/128 3,337,404 8/1967 Polli et a1... 424/153 OTHER SOLUTES To A MAMMAL 3,356,570 12/1967 Butcher 424/153 [75] Inventor: Beverly L. Reynolds, Dallas, Tex. 3,676,553 7/1972 Reynolds 424/128 [73] Assignee: Cybersol, Inc., Dallas, Tex.

Primary Examiner-Stanley .1. Friedman 1 Nonce 3223 3 83 33 s fi g 989 Attorney, Agent, or FirmGiles C. Clegg, Jr.

has been disclaimed.

[22] Filed: July 21, 1972 ABSTRACT [21] Appl. No.: 273,849 Drugs, nutrients and other solutes are administered to fixed and mobile tissue cells of the mammal in an t t L 1971, abandoned, which is a continuation-in-part of 2 :g 3 n/M/L 5 5 p Ser, No. 885,295, Dec. 15, 1969, Pat. No. 3,676,553. taming about 3 8 g and about 3 8 HPO, and/or 80 and/or acetate, and/or gluco- [52] l P 1 nate; the solution having a pH of about 7.4 to 8.4 and 51 I t Cl 2 AGIK 27/00 an osmolality of about 170460, preferably about I54 162 260340, more preferably about 290-310, and most 1 0 earc 424/93; preferably 300 milliosmols per liter. The composition can contain other additives, e.g. stabilizing agents, and the solutes to be carried, e.g., antibiotics, vitamins, nu-

[56] uNlTE g ggafris gg rENTs trients, and other solutes.

233,063 10/1880 Boughton 424/128 6 Claims, N0 Drawings METHOD FOR ADMINISTERING WATER SOLUBLE DRUGS, NUTRIENTS AND OTHER SOLUTES TO A MAMMAL This application is a continuation-in-part of my eartabolism so as to attain the same effect as obtained by the incorporation of HC ion itself. When, however, a large amount of blood is lost as in the cases of serious wound and surgical operation, the metabolitic function lier copending U.S. Pat. application, Ser. No. 149,081 5 of the living bodies is considerably depressed so that filed June l, 1971, entitled Method for Administering the immediate formation of HC0 is difficult. More- Water Soluble Drugs, Nutrients and Other Solutes to a over, any unfavorable side effect is unavoidable, ba- Mammal", now abandoned which is in turn a continuucause the composition of the artificial transportation tion-in-part of my earlier filed US application Ser. No. liquids is anyhow different from that of blood plasma 885,295. filed Dec. 15, 1969, now US. Pat. No. it lf, Considering the above situation, the appearance of a BACKGROUND OF THE INVENTION transportation liquid containing HC0 ion itself and ha th s'm l This invention relates to methods for transporting 1 flar compositlon to extmceuilldr with a high stability has been desired, because of its extreme and dispersing pharmaceutical preparations within the I usefulness and advantage. As the results of the extenbody of a living animal. More particularly, it relates to Sive stud they h b l d th the use of aqueous solutions as carriers for physiologe een comp f present ically active substances as pharmaceutical preparalrecte to Sue transportatlon tions, vitamins, protein, blood cells, etc., without dele- The composltlon of the Present invention coffeteriously affecting the physiologically active substance sponds to that of the average extracellular SolutiOn of d hi h may b dil di d i th li i b d various mammals including human beings determined without causing undesirable chemical or physiological y the h rm ynamic quation and the computor reactions. analysis and confirmed by the experimental test. There- Various artificial carrier media are commonly used fore, the presence and numerical limitation Of the said for parenterally administered ompositions Typical Z5 lOnS 8T6 essential. If anyone Of these factors iS not satiscarrier media are such compositions as water for injecthen th a an etween the extracellular solution, U.S.P., lactated Ringers solution, normal saline, tion and the intracellular solution is broken so that the and dextrosesaline. While these solutions can be used concentrations of the solutes in the intracellular soluas biologically and chemically inert solvents or carriers tion are varied to afford an unfavorable influence on f most h i l i ll ti ub t they a living bodies which received the composition. Thus, it have undesirable physiological effects in the living may be said to be the first composition similar to extrabody in which they are used, and may be quite ineffeccellular containing HCO ion in a stable state, which tive in transporting the Substances through the extrais an ideal carrier for a pharmaceutical Substance of pa cellular water of the patient to the desired location in renteral use. either the intracellular water or extracellular water or in preparation of the composition of the invention both-furtherfnfjrei Q Y P Gamer 9 the order of addition of the essential ion sources to are slightly acidic, causing inactivation or deterioration water is not important and may be Optional of the substance prior to lnJCCtlOn into the living body. t The compositions of known commercially available ar- Any pharmacfif'lfucal subsiance m mcorpfrated tificial transportation liquids are shown together with 40 the Composltlof P h mveniloni q t it does the Composition of human plasma in the foiiowing not cause any precipitation of said essential ions. ble, from which it is seen that HCOfion present in a The composition of the invention may be used as a relatively high concentration in human plasma does not dilution liquid for concentrated blood plasma. exist in the artificial transportation liquids: Effectiveness of the substance at the presumed site of Approximate Cations (MEq/L) Anions (mEq/L) Solution pH Na K Mg Ca Cl Phosphate HCO Acetate Gluco- Lactate nate Human Plasma 7.4 140 4-5 2-3 5 102 2.5 27 5 Normosol R* pH 7.4 7.4 I40 5 3 98 27 23 Lactated Ringer's 6.5 130 4 3 109 23 Normal Saline 5.4 154 154 Dextrose 5% in saline 4.6 154 154 Polysal** 6.2 140 10 3 10 103 Plasma-Lyte*** 6.2 140 l0 3 5 103 47 8 Trademark of Abbott Laboratories. North Chicago. Illinois "Trademark of Cutter Laboratories. Berkeley [0. California "Trademark of Baxter Laboratories. Inc. Morton Grove. Illinois The main reason why the artificial transportation liqaction is dependent upon concentration, provided its uids do not contain 'HC03' ion is due to the formation chemical and pharmaceutical activity has not been reof C0 ion in the essential heating and sterilizing duced Significantly during Solutionm and Storage the gtgps the C03 ion being combined ca ion o aqueous medium 01' its movement from the 51118 of th i t gi an i l bl lt as a i it t administration to the site of action. Transport media Thus, as een in the above Table, the artificial transporwhich enhance transit time after administration Will, Of

tation liquids contain various organic acids (e.g. acetic acid, gluconic acid, lactic acid) being participated in the formation of HCO ion in the consequence of mecourse, provide a higher concentration of substance whose activity is greater than those media whose composition does not permit rapid transit time.

Movement of the substance and medium is dependent upon its osmolality or osmolal pressure, e.g., the pressure produced in a solvent system by the action of the total number of particles dissolved in that solvent. In a living animal the solvent system is, of course, water.

The mean osmolality of extracellular water in humans and most animals is at or near 300 milliosmols per liter (mosm/L).

The pH of previous artificial carrier media has frequently been below that of the mean of animal body. Moreover, osmolality and chemical composition of the carrier with respect to the osmolality and chemical composition of the animal plasma or extracellular water has been largely ignored.

It is generally understood that the pH of the aqueous medium used as a carrier should be within the general limits observed and reported in human and animal patients in healthy and diseased condition. This range is reported to be between 6.7 and 7.8. In the diseased state either human or animal bodies frequently form compounds in both the extracellular water and the intracellular water which have acidic properties, thus altering the pH of the extracellular water toward acidity from the physiological mean of 7.2 to 7.6. Therefore, the pH of the transport medium should be increased beyond the general limits of the extracellular water in either human or animal patients, to 8.1 or greater, to compensate for the acidic tendencies. In either human or animal patients whose pH in the extracellular water does not alter in the acidic direction, a pH of 8.1 in the aqueous medium is tolerated without incident unless overloaded with the aqueous medium to a excess of the total body weight or greater. Establishing the pH range in this manner inhibits inactivation of the carried additive while in storage in the aqueous medium before administration. Obviously, to be effective the additive must be active within the limits of pH of the extracellular water in human and in animals. Conventional solutions have largely ignored the distribution mechanism involved in distributing the artificial carier media, and the included substance, within the living body.

Notwithstanding, the desirability of utilizing pH levels established in accordance with the foregoing discussion, it should be understood that the carrier must have a pH compatible with the additive being administered, and that some additives require fairly low pH carriers. Furthermore, pH is not as critical a parameter of the carrier solution as other parameters, because the relatively small amounts of carrier liquid added to the body fluid system are readily buffered by the strong self buffering properties of the blood stream and total body fluid system.

SUMMARY OF THE INVENTION In accordance with this invention solutes to be administered are solubilized in a water solution containing selected ones of the solutes of extracellular water. The solution contains specific cations and anions in percentages and concentrations to produce an osmolality within the range of 170 to 416, preferably 260-340, more preferably 290-310 and most preferably 300 milliosmols per liter and a pH preferably in the range of about 7.0-8.8, more preferably about 7.5-8.5 and most preferably 8.1.

In the preferred embodiment the solution contains about 72-200 millimols per liter of sodium ion (Na*),

7-25 millimols per liter potassium ion (K 3-8 millimols per liter magnesium ion (Mg*'), 308 millimols per liter phosphate ion (HPOf) or sulfate ion (SOf) or gluconate, or acetate, 12-28 illimols per liter bicarbonate ion CHCOJ) and -195 millimols per liter chloride ion (Cl The mol quantity of each solute, as chemical compounds, is adjusted to achieve mass and velocity balance, which results from adjusting the quantities of major, intermediate and minor solutes such that (l) the ratio of the mass-velocity product of the major to each intermediate solute is near equal, and (2) the quotient of the ratios of the major to minor and intermediate to minor is near equal to the ratio of the major to intermediate solute. Mass-velocity product balance produces a most nearly efficient mix of solutes with respect to solute activity, for the percent of each solute by weight should be near equal to its percent of the cumulative mass-velocity product. Since the combination of identical solute compounds will provide greater solute ion activity, millimolality by weight is optimally near millimolarity by activity.

Furthermore, since intracellular pH is determined by changes in venous CO and is not directly related to lactate concentrations in extracellular water, bicarbonate ions are included in the solution which control intracellular as well as extracellular pH.

DESCRIPTION OF PREFERRED EMBODIMENT The composition of the preferred embodiment of the carrier medium of the invention is set forth in the following table.

The media contains sodium as the principal major cation and chloride as the principal major anion. Potassium and carbonate are the intermediate cation and anion. These ions are selected because their coefficient of mobility with the hydrogen ion (H+) and hydroxyl ion (OI-l") are higher with respect to other solutes in any given situation. Magnesium is the principal minor solute cation and the phosphate ion and/or sulfate ion and/or acetate and/or gluconate are the principal minor solute anions. the magnesium, phosphate, sulfate, acetate and gluconate ions assist in stabilization of solute velocity, hence distribution, in the extracellular water.

The solution contains true bicarbonate solute, rather than substituted organic anionic solutes as in conventional solutions. The bicarbonate ion is used because only CO gas. H+and OH-ion solutes move rapidly in all water components. Accordingly, by adjusting the mix of solutes with respect to solute activity as set forth hereinabove, the osmolality of the solution can be accurately controlled to establish partition rates between the total blood volume and the interstitial water in quantities nearly equal to their percentages in the normal body, i.e., 25% and 75%, respectively. By controlling osmolality of the solution as described above the additive carried in the solution is readily dispersed throughout the extracellular water and intracellular water in the ratio in which water systems would be distributed in the normal body without causing imbalance of any water system.

The osmolality of the solution may be varied as desired within a range from about 170 to about 460. Where equiosmolality is desired, i.e., where movement of the carrier media and additive through the total blood volume and interstitial water is desired in ratios nearly equal to normal, osmolality of the carrier media should preferably be adjusted to range from 290 to 310, preferably about 300, the mean osmolality for the extracellular water. It should be noted, however, that osmolality may be varied as desired and the carrier medium, along with its additive, may be concentrated in the intracellular water of either mobile or fixed cell systems, therefore aiding in direct treatment at the desired site. Accordingly, solutions having a total osmolality of less than about 260 can be designed to move into the cells and such solutions are useful in treatment of situations in which water losses have been chronic. Likewise, if the total osmolality is adjusted to about 320 or greater, the solution can be designed to dehydrate the cells.

It will be observed that since physiologically active substances dispersed in the solution described herein may be readily moved into the intracellular water, the effect of such additive will be greatly enhanced because of the low excretion rate of the additive. Furthermore, since the osmolality of the solution is preferably adjusted to near 300, the carrier media, and therefore the additive, is rapidly dispersed throughout the water systems of the body without adversely affecting normal balances. Furthermore, since the carrier medium is preferably adjusted to pHs on the slightly basic side, as contrasted to the normal acidic qualities of conventional solutions, the additive is, in most cases, less likely to suffer damage or inactivation during preparation or storage. i

The ionic concentrations set forth above are achieved in an aqueous solution by'dissolution of solutes selected on the basis of activity coefficient and mass-velocity product. However, when the solution is itself dispersed in the animal body, further dilution 'of the solution occurs. Therefore, solutes must be selected which have a high activity coefficient so that upon fur- EXAMPLE I Solute Concentration Mass-Velocity Activity (mM/L) Product Coefficient A. NaCl ll3 13.25 0.85 B. KCL l4 2.26 0.93 C. NaHCO l8 2.l2 0.97 D. MgSO, 5 0.51 0.75

In order to maintain the desired ionic ratios in the solution after dispersion within the body fluids, the concentration ratios of the solutes should be approximately as follows: (referring to the above example) Conc.A Conc..4 Conc.B Conc.C

The pH of solutions such as described above may be controlled as desired. Usually, the carrier should be slightly basic, in the range of about 7.8 to 8.1 and may be buffered to maintain approximately the desired pH.

Since the solution is normally slightly basic, the inactivation of many pharmaceutical preparations by dissolution in the carrier is avoided, thereby increasing shelf life and rendering the additive more active when used. For example, many antiobiotics are very sensitive to acid conditions but can withstand basic solutionswithout being deleteriously affected.

The carrier solution described may be used as a medium for transporting any substance which is soluble in water. The additive, when dissolved in the carrier medium, has little effect on the properties of the carrier solution, and moves with the solution throughout the body systems. Molecules as large as albumin dispersed in the carrier medium have been found to readily move with the solution throughout the body water systems as rapidly as smaller ions.

EXAMPLE II A solution having the concentration indicated under the heading Most Preferred in the foregoing table in the description of the preferred embodiment, and having an adjusted pH of about 7.5 was used as an experimental carrier for packed red blood cells and plasma protein in the course of a hemophiliac donor program. The carrier was employed to facilitate the return to the donors of the packed red blood cells (erythrocytes) and plasma protein following extractionfrom the donated whole blood of the components needed for the donor program. Also employed for this purpose in the program was lactated Ringers solution, a prior art car'- rier.

The results presented in the table below are typical of those obtained in over six thousand applications. The comparison between the carrier solution employed in accordance with the present invention and the lactated Ringers solution appearing in the table is of high validity because the data is derived from successive tests done with'one carrier solution and then the other on the same donors during the program, thus eliminating variations which might flow from differences between individuals.

As can be seen from the Table below, equal volumes of packed blood cells and plasma protein and carrier were employed for the solutions being returned to the donors.

A hematocrit measurement was made on each donor just prior to his donation of whole blood, and at a fixed Carrier of Lactated Solution Transferred invention Ringer's Quantity of solution administered (m1) 250 250 Quantity of Packed Cells and Protein administered (m1) 250 250 75 Decrease in Hematocrit Pre-to Post-Transfusion 4 l5 71 Decrease in Total Protein Pre-to Post-Transfusion -25 From the foregoing, it can be seen that carrier solutions employed in accordance with the invention produce a near-normal condition in the donors, while the prior art Lactated Ringers carrier solution leaves the donors in an abnormal or disabled condition with respect to two important blood parameters. As a practical matter, it is possible to have a donor give blood much more frequently when the packed cells and plasma protein are returned to him with the carrier of the present invention because the recuperative time for restoration of his blood parameters to normal condition in readiness for the next donation is much reduced.

EXAMPLE 111 Another series of tests was run to determine and illustrate the effect of the carriers of the present invention upon the potency of a physiologically active material, namely an antibiotic, as compared to prior art carrier or transport solutions.

Three carrier solutions were employed in the tests. The first was a solution having the concentration indicated under the heading Most Preferred in the foregoing table in the description of the preferred embodiment, and having an adjusted pH of about 7.5. The second was Lactated Ringers solution, and the third was a dextrose solution in water. A penicillin-type antibiotic, having the tradename Methicillin, was employed for the tests. Ten milligrams of Methicillin was added to 500 milliliters of each of the three carrier solutions. The mixtures so obtained were incubated for 30 minutes at 37C. One milliliter from each of the three mixtures, after incubation, was mixed with one milliliter of staphylococcus aureus, coagulase positive, thioglycollate broth and those mixtures were incubated. After one hour of incubation and after 24 hours of incubation, identical aliquots were removed and cultured on bacteriological discs for 24 hours. In the Table appear-- ing below, the colony count per milliliter is reportedfor each of the three solutions for the two periods of incubation.

Solutions Used Hours for incubation in Test Media From the foregoing test results, it can be seen that the antibiotic retained its physiologically active properties much more effectively in the carrier of the present invention than in the prior art carriers used for comparison. This circumstance provides a great advantage in the use of the carriers of the invention in connection with antibiotics, because the activity of the antibiotic is maintained and delivered to its point of use in the body undegraded by the carrier.

What is claimed is:

l. A method for administration of solutes to a mammal wherein the solutes are mixed in an aqueous solution, characterized by the aqueous solution having an osmolality in milliosmols/liter of 170 to 460, preferably 260 to 340, more preferably 290 to 310 and most preferably 300, a pH of 7.0 and 8.8 and more preferably between 7.5 and 8.5, and most preferably 8.1 and formed of compounds having an activity coefficient in the solution of at least 0.8 at 37C to provide concentration in milliosmols/liter of Na 75-200, preferably 85-150, more preferably 127-135 and most preferably 131, of K 7-25, preferably 10-20, more preferably 13-15 and most preferably 14, of Mg 3-8 and preferably 5, of HPO4= or S0 or Acetate or Gluconate 3-8 and preferably 5, or HCO 12-28, preferably 10-30, more preferably 16-20 and most preferably 18 and of Cl- -195, preferably 85-185, more preferably 124-130 and most preferably 127.

2. A method in accordance with claim 1 in which said solute is packed blood cells of the mammal.

3. A method in accordance with claim 1 in which said solute is plasma protein of the mammal.

4. A method in accordance with claim 1 in which said solute is an antibiotic.

5. A method in accordance with claim 4 in which said antibiotic is a penicillin.

6. A method for administration of solutes to a mammal wherein the solutes are mixed in an aqueous solution, characterized by the aqueous solution having an osmolality in milliosmols/liter of 170 to 460, preferably 260 to 340, more preferably 290 to 310 and most preferably 300, and formed of compounds having an activity coefficient in the solution of at least 0.8 at 37C to provide concentration in milliosmols/liter of Na -200, preferably -150, more preferably 127-135 and most preferably 131, of K 7-25, preferably 10-20, more preferably 13-15 and most preferably 14, of Mg 3-8 and preferably 5, of HPO, or S04 or acetate or gluconate 3-8 and preferably 5, of HCO3 12-28, preferably 10-30, more preferably 16-20 and most preferably 18 and of Cl 70-195, preferably 85-185, more preferably 124-130 and most preferably 127, and a pH insufficiently different from that of the fluid system of the mammal to which it is to be administered to materially alter the system pH. 

1. A METHOD FOR ADMINISTRATION OF SOLUTES TO A MAMMAL WHEREIN THE SOLUTES ARE MIXED IN AN AQUOUEOUS SOLUTION, CHARACTERIZED BY THE AQUEOUS SOLUTION HAVING AN OSMOLALITY IN MILLIOSMOLS/LITER OF 170 TO 460, PREFERABLY 260 TO 340, MORE PREFERABLY 290 TO 310 AND MOST PREFERABLY 300, A PH OF 7.0 AND 8.8 ND MORE PREFERABLY BETWEEN 7.5 AND 8.5, AND MOST PREFERABLY 8.1 AND FORMED OF COMPOUNDS HAVING AN ACTIVITY COEFFICIENT IN THE SOLUTION OF AT LEAST 0.8 AT 37*C TO PROVIDE CONCENTRATION IN MILLIOSMOLS/LITER OF NA* 75-200, PREFERABLY 85-150, MORE PREFERABLY 127-135 AND MOST PREFERABLY 131, OF K* 7-25, PREFERABLY 10-20, MORE PREFERABLY 131, OF K* 7-25, 14, OF MG** 3-8 AND PREFERABLY 5, OF HPO4= OR SO4= OR ACETATE OR GLUCONATE 3-8 AND PREFERABLY 5, OR HCO3 12-28, PREFERABLY 10-30, MORE PREFERABLY 16-20 AND MOST PREFERABLY 18 AND OF CI-70-195, PREFERABLY 85-185. MORE PFEFERABLY 124-130 AND MOST PREFERABLY
 127. 2. A method in accordance with claim 1 in which said solute is packed blood cells of the mammal.
 3. A method in accordance with claim 1 in which said solute is plasma protein of the mammal.
 4. A method in accordance with claim 1 in which said solute is an antibiotic.
 5. A method in accordance with claim 4 in which said antibiotic is a penicillin.
 6. A method for administration of solutes to a mammal wherein the solutes are mixed in an aqueous solution, characterized by the aqueous solution having an osmolality in milliosmols/liter of 170 to 460, preferably 260 to 340, more preferably 290 to 310 and most preferably 300, and formed of compounds having an activity coefficient in the solution of at least 0.8 at 37*C to provide concentration in milliosmols/liter of Na 75-200, preferably 85-150, more preferably 127-135 and most preferably 131, of K 7-25, preferably 10-20, more preferably 13-15 and most preferably 14, of Mg 3-8 and preferably 5, of HP04 or S04 or acetate or gluconate 3-8 and preferably 5, of HC03 12-28, preferably 10-30, more preferably 16-20 and most preferably 18 and of C1 70-195, preferablY 85-185, more preferably 124-130 and most preferably 127, and a pH insufficiently different from that of the fluid system of the mammal to which it is to be administered to materially alter the system pH. 